Can anybody help with this dilemma.
During PQ of the autoclave cycle one of the requirements is that the Fo specification must be met.
We are using a Kaye 2000 which calculates the lethality. However, there is some confusion at what point the Fo is taken.
1. Some suggest at the end of the exposure phase (end of 20 minute hold time)
2. Some suggest at the end of the autoclave cycle, as the Fo is still accumulating. (The Kaye recording is stopped 25 minutes after the end of the exposure phase)